Universal Amino
8
May/090
May/090
Dna sequences 5" to 3"?
Help got this wrong in a recent test and cant work out or understand the correct answer
Using the DNA sequence below
3'TACTACTAGCCGCGCCTCTCTCCC5'
Transcribed Strand (template)
write down the 5’ to 3’ sequence of mRNA it encodes.
(i.e. the question marks below)
5'????????????????????????3'
Using the table of the Universal Genetic Code, translate this sequence of codons from within the mRNA to yield the amino acid sequence.
The first two answerers are incorrect. Amy above is right.
To determine the mRNA sequence, keep in mind these base-pairing rules:
A (adenine) --- U (uracil)
G (guanine) --- C (cytosine)
Note that adenine in DNA binds to uracil in RNA. (RNA does not have Thymine). And, Thymine in DNA binds to adenine in RNA.
Hence, given the template DNA strand, the complementary mRNA sequence is as follows:
3' TAC TAC TAG CCG CGC CTC TCT CCC 5'
5' AUG AUG AUC GGC GCG GAG AGA GGG 3'
Each codon on the mRNA molecule specifies a particular amino acid. Hence, using the genetic code, the amino acid sequence is as follows:
Methionine (start) - Methionine - Isoleucine - Glycine - Alanine - Glutamic Acid - Arguinine - Glycine
You use this genetic code to determine the amino acid sequence:
http://img.tfd.com/mgh/ceb/thumb/Universal-standard-genetic-code.jpg
I hope this helps
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