Creatine Alpha
Dec/100
Semi-in vitro repair assay in cell extracts?
Having trouble doing repair assays in cell extracts. Baically, I am mixing cell extract, plasmid DNA (with damage), and buffer containing: Hepes, MgCl2, DTT, ATP, dNTPs, hot dCTP (alpha-32P), phosphocreatine, creatine phosphokinase, and KCL/EDTA/glycerol. Is there a an efficient way to test the activity of my cell extracts?
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